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SRX10008471: GSM5060550: SSc_Biopsy_059; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 38.4M spots, 1.9G bases, 651.3Mb downloads

Submitted by: NCBI (GEO)
Study: Examining gene expression profile from varying preparations of fibrotic lung tissue
show Abstracthide Abstract
Gene expression of human lung tissue bronchoalveolar lavage (BAL), whole tissue biopsy, and digested single cell suspension of lung tissue from control and ILD samples. Overall design: Gene expression profiles were captured from various samples derived from human lung tissue explants. Bronchoalveolar lavage, whole tissue biopsy, and a single cell suspension derived from digested lung tissue were prepared and evaluated via RNA-seq. This allowed for evaluation of sample preparation, and retention of singal in digested samples, to ensure that there was not significant loss of signal due to incomplete digestion or bias of preparation protocol.
Sample: SSc_Biopsy_059
SAMN17764676 • SRS8177532 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using the Qiagen RNeasy kit. Libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit.
Experiment attributes:
GEO Accession: GSM5060550
Links:
Runs: 1 run, 38.4M spots, 1.9G bases, 651.3Mb
Run# of Spots# of BasesSizePublished
SRR1361507438,350,3021.9G651.3Mb2021-03-09

ID:
13135885

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